Resources: Technical Paper

Techniques for the Determination of Clutch Parameters in Darters and Minnows:
A Proposal for Standard Methods by David C. Heins

Contents
Introduction General Description of Ovaries and Ova Two Former Techniques and Their Associated Views of Ovum Production Using Only "Mature Ova" Using Both "Immature Ova" and "Mature Ova" Toward Standard Methods The Proposed Standard Methods: Elements of Both Former Approaches Clutch Size Ovum Size: Diameter and Mass Relative Clutch Mass (RCM) Drying Temperatures Weighing Tissues Comparison of Proposed Standard Methods with Prior Methods Fixation and Preservation References Definitions Table 1. Stages of Ovarian Condition in Adult Darters Figures Figure 1. Ventral View of Ovaries from Mature (MA) Female of Etheostoma caeruleum Figure 2. Ventral View of Ovaries of Mature (MA) Female of Etheostoma caeruleum Figure 3. Ventral View of Ovaries of Ripening (MR) Female of Etheostoma caeruleum Figure 4. Ventral View of Ovaries of Ripe (RE) Female Etheostoma caeruleum Figure 5. Ventral Views of Ovaries from Mature (MA), Ripening (MR), and Ripe (RE) Females of Etheostoma caeruleum Figure 6. Variation in Mature Ovaries of Etheostoma caeruleum Females Figure 7. Variation in Ripening Ovaries of Etheostoma caeruleum Females Figure 8. Variation in Ripe Ovaries of Etheostoma caeruleum Females Figure 9. Mature, Ripening and Ripe Ovaries of Etheostoma simoterum Female

Contents